• Multi-Purpose, High Purity
• Enhanced Resolution and Clarity
• Reduced Background
• Low EEO / Increased Electrophoretic Mobility
• RNase-, DNase- and Protease-Free
• High Hysteresis / Improved Thermal Stability
Agarose LE is a highly purified agarose, used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols. It's refined using a process that excludes organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150bp to 6kbp).
Its low EEO (Less than or Equal to 0.13mr) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counter flow) that can result from the presence of excessive sulfate-rich negative ions.
Benchmark Agarose LE easily soluble and free of nucleases. It is highly transparent (forms a clear, colorless solution at 1g: 100mL H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.
Formulated for high gel strength and integrity, Agarose LE exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added.